Services

3D

WHY 3D?

3D cultures are better in mimicking cells’ natural environment and by doing so, they provide more reliable platform for all life science research. Today more than 95% of the drugs that gave positive results in laboratory 2D test are rejected in animal study or clinical trials. One of the reasons behind this problem is that today’s standard procedures involve almost entirely 2D cell cultures which are often misleading. On the other hand in 3D cell cultures, cells grow in a physiological way, it brings back their gene expression profile to the physiological state and as a result these cell respond as if they would live in an organism.

WHY LifeGel ?

LifeGel is delivered on standard well plates in “ready to use” form. Only thing you have to do is to open the plate and use it! Our hydrogel is highly reproducible, free of contaminations and costeffective, allowing for easy testing and reliable results.

OUR SERVICES

With 3D cultures you can optimize cell growth and adjust it to your needs, by using different types of LifeGel you can obtain different growth conditions (type of spheroids or whether they grow on top of hydrogel or within). Real Research provides LifeGel for your experiments. We can also optimize the gel for specific cell type or experiment of your choice. All you have to do is send us the cell bank and we will compare several subtypes of LifeGel, after which you’ll receive full – detailed report and you’ll be able to chose the option that best suits your needs. We are also prepared to do several downstream analysis, including (but not limited to):

→ Comparison of 2D vs. 3D

Comparison of structures by means of morphology or proliferation; we can bank the cells after culture and send them to you for further analysis along with the pictures and full – detailed report; suitable also for primary cell cultures.

→ Long – term 3D cultures

Long – term cultures enable you to see how your cells behave during prolonged growth in three-dimensional conditions. The assay comes in handy while testing different drug concentrations/time periods. It is also a great tool for obtaining cells that are sensitive or resistant to a specific compound.

Image 1: 3D culture of breast cancer cells on LifeGel. Culture after: A- 14 days, B- 17 days, C- 20 days

→ In vitro tube formation assay

It is an assay used to determine the ability of endothelial cells to create capillary – like structures during desired condition. In vitro tube formation assay is a great tool for examining the effect of studied compounds on tube formation. Particularly used in oncology – very useful for testing drugs that affect tumor angiogenesis.

→ Aortic ring assay

Aortic ring assay is a useful tool for examining in vitro angiogenesis using aortic tissue sample. Impossible to perform on most of the commercially available 3D platforms.

→ Intestinal permeability assay

Especially useful for testing drugs intended for oral consumption in order to determine the adsorption kinetics.

→ Customized service

Other assays can be discussed individually.

Image 2: A: Tube formation assay on LifeGel (HUVEC), B: Aortic ring assay on LifeGeL

We are at your service: (+48) 603-984-577

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