3D cultures are better at mimicking the cells’ natural environment, which means they provide a more reliable platform for all life science research. Today, more than 95% of the drugs that give positive results in laboratory 2D tests are rejected in animal studies or clinical trials. One of the reasons behind this problem is that today’s standard procedures involve almost entirely 2D cell cultures, which can often provide misleading results. On the other hand, in 3D cell cultures, cells grow in a physiological way, restoring their gene expression profile to the physiological state, which means that the cells respond as they would in a living organism.

WHY LifeGel ?

LifeGel is supplied on standard well plates in a “ready-to-use” form. All you have to do is to open the plate and start to use it! Our hydrogel is highly reproducible, free from contamination and cost-effective, allowing for easy testing and providing reliable results.


With 3D cultures, you can optimize cell growth and adjust it to your needs. The different types of LifeGel will enable you to obtain different growth conditions (in terms of the type of spheroids which form or whether they grow on top of the hydrogel or within). Real Research provides LifeGel for your experiments and we can also optimize the gel for a specific cell type or experiment of your choice. All you have to do is send us the cell bank and we will compare several subtypes of LifeGel, after which you’ll receive a full detailed report enabling you to choose the option that best suits your needs. We are also prepared to do a number of downstream analyses, including (but not limited to):

→ Comparison of 2D vs. 3D

A comparison of structures by means of morphology or proliferation – we can bank the cells after culturing and send them to you for further analysis along with pictures and a full detailed report. This is also suitable for primary cell cultures.

→ Long – term 3D cultures

Long-term cultures enable you to see how your cells behave during prolonged growth in 3D conditions. This assay comes in handy while testing different drug concentrations/time periods and it is also an excellent tool for obtaining cells that are sensitive or resistant to a specific compound.

Image 1: 3D culture of breast cancer cells on LifeGel. Culture after: A- 14 days, B- 17 days, C- 20 days

→ In vitro tube formation assay

An assay used to determine the ability of endothelial cells to create capillary-like structures in the desired condition. An excellent tool for examining the effect of studied compounds on tube formation, it is often used in oncology as it is very useful for testing drugs that affect tumor angiogenesis.

→ Aortic ring assay

An aortic ring assay is a useful tool for examining in vitro angiogenesis using aortic tissue samples, which is impossible on most commercially available 3D platforms.

→ Intestinal permeability assay

Especially useful for testing drugs intended for oral consumption in order to determine the adsorption kinetics.

→ Customized service

Other assays can be discussed individually.

Image 2: A: Tube formation assay on LifeGel (HUVEC), B: Aortic ring assay on LifeGeL

We are at your service: (+48) 603-984-577

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